| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Leptospira biflexa | GTGGTTCGGAAGCAGGGAT | ACAATGGCGATCGGAGCA | 59.70 | 59.42 | 268 | 138 |
100.00%
|
100.00%
|
| Primer 22 | Leptospira biflexa | AACGGATGCATGGCCACA | GGTTCCAAAGCCAAACAGCA | 59.96 | 59.54 | 204 | 137 |
100.00%
|
100.00%
|
| Primer 23 | Leptospira biflexa | AACGGATGCATGGCCACA | TGGTTCCAAAGCCAAACAGC | 59.96 | 59.54 | 205 | 137 |
100.00%
|
100.00%
|
| Primer 24 | Leptospira biflexa | AACGGATGCATGGCCACA | TGGACCCCTGTACACACAAG | 59.96 | 59.24 | 284 | 137 |
100.00%
|
100.00%
|
| Primer 25 | Leptospira biflexa | AACGGATGCATGGCCACA | TTGGACCCCTGTACACACA | 59.96 | 58.07 | 285 | 137 |
100.00%
|
100.00%
|
| Primer 26 | Leptospira biflexa | AACGGATGCATGGCCACA | TTGGTTCCAAAGCCAAACAGC | 59.96 | 60.13 | 206 | 137 |
100.00%
|
100.00%
|
| Primer 27 | Leptospira biflexa | AACGGATGCATGGCCACA | GCAATGTTTTCGCTGGTTGG | 59.96 | 58.86 | 187 | 137 |
100.00%
|
100.00%
|
| Primer 28 | Leptospira biflexa | TTCCATCCCCTCCCGATGT | TTGACCACTGCCTGCAAAA | 60.00 | 58.11 | 152 | 137 |
100.00%
|
100.00%
|
| Primer 29 | Leptospira biflexa | TTCCATCCCCTCCCGATGT | TTTGACCACTGCCTGCAAA | 60.00 | 58.11 | 153 | 137 |
100.00%
|
100.00%
|
| Primer 30 | Leptospira biflexa | TGCGATCGGAATGCCACA | CCAATCCACACTGCGGAGT | 59.73 | 60.00 | 172 | 137 |
100.00%
|
100.00%
|